Nuclear genome size and cytotype analysis in Agave parviflora Torr. subsp. flexiflora Gentry (Asparagales, Asparagaceae)

Autores: 
G. Palomino
J. Martínez
I. Méndez
Cepeda-Cornejo
R. Barba-González
B. Rodríguez-Garay
Revista y/o libro: 
Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics
Editorial: 
CIATEJ
Volumen: 
68(3), 159-168
Año: 
2015
Sinopsis: 
This study presents the cytogenetic characterization by karyotyping and a determination of the DNA content by flow cytometry of Agave parviflora subsp. flexiflora from Bacadehuachi to Nacori Town, Sonora, Mexico. The analyzed plants were diploids (2n = 2x = 60) The cytotype observed in wild adult plants was 44m + 4sm + 10st + 2t, the cytotype found in plants grown from seeds was 48m + 8st + 4t and the cytotype displayed by bulbils was 44m + 2sm + 14st. Differences in the amount of nuclear 2C DNA = 8.20 pg in diploid wild adult plants and 8.21 pg in bulbils were significantly different when compared to 2C DNA = 8.07 pg of diploid plants grown from seeds. The different types of plants displayed 1.7% variation in the 2C DNA content and the mean 2C DNA content was 8.16 pg; 1Cx value = 4.08 pg.
Resumen: 
This study presents the cytogenetic characterization by karyotyping and a determination of the DNA content by flow cytometry of Agave parviflora subsp. flexiflora from Bacadehuachi to Nacori Town, Sonora, Mexico. The analyzed plants were diploids (2n = 2x = 60) The cytotype observed in wild adult plants was 44m + 4sm + 10st + 2t, the cytotype found in plants grown from seeds was 48m + 8st + 4t and the cytotype displayed by bulbils was 44m + 2sm + 14st. Differences in the amount of nuclear 2C DNA = 8.20 pg in diploid wild adult plants and 8.21 pg in bulbils were significantly different when compared to 2C DNA = 8.07 pg of diploid plants grown from seeds. The different types of plants displayed 1.7% variation in the 2C DNA content and the mean 2C DNA content was 8.16 pg; 1Cx value = 4.08 pg.