Physical mapping of 5S and 18S ribosomal DNA in three species of Agave (Asparagales, Asparagaceae).

Autores: 
Gomez-Rodriguez VM.
Rodriguez-Garay B.
Palomino G.
Martínez J.
Barba-Gonzalez R.
Revista y/o libro: 
Comparative Cytogenetics
Volumen: 
7(3), 191-203
Año: 
2013
Sinopsis: 
Cytogenetic analysis was carried out in Agave tequilana “Azul”, A. cupreata and A. angustifolia showed that in all species the diploid chromosome was 2n=60, with bimodal karyotype composed of five pairs of large chromosomes and 25 pairs of small chromosomes. Furthemore different karyotypical formulae as well as secondary constriction in a large chromosome pair were found in all species. Fluorescent in situ hybridization (FISH) was used for physical mapping of 5S and 18S ribosomal DNA (rDNA). All species analyzed showed that 5S rDNA was located in both arms of a small chromosome pair, while 18S rDNA was associated with secondary of large chromosome pair. Data of FISH analysis provides new information about the position and number of rDNA loci.